ABSTRACT
Objective To study the color of Ruyi Jinhuang Powder (RJP) placebo by using computer color matching technology and achieve fast preparation of RJP placebo. This work provided a new method to simulate the color of Chinese medicine compound placebo. Methods Using the RJP placebo as an example, computer color matching technology was used to establish a mathematical model to correlate the placebo color parameter L, a*, b* value and the colorant concentration. For a measured medicine compound color, the placebo colorant concentration can then be calculated from the model through data fitting and Newton iterative method. The model accuracy was validated using the color comprehensive evaluation index (ΔE). Results The color parameter L, a*, b* of RJP placebo was 68.302 5, 4.079 5, and 34.484 0. The mass fraction of lemon yellow, amaranth, and blue was 0.837 3%, 0.045 8%, and 0.008 5%. The ΔE value between RJP and its placebo was 2.750 0 ± 0.353 6, and there was no obvious visual difference between the medicine compound and its placebo. Conclusion Computer color matching technology can be used to simulate the color of RJP placebo, and can be widely used in the preparation of Chinese medicine placebo.
ABSTRACT
Based on DPPH method, the antioxidant activities of Shenqi Tongmai Yizhi particles with different extraction processes were compared. The contribution to the anti-oxidant capacity in vitro was explored by means of grey relational analysis on different chemical compositions in the fingerprint. The results showed that the IC₅₀ concentration values of water extract, water extract from alcohol precipitation, alcohol extract, and alcohol and water extract were 0.801 4, 0.859 1, 0.796 1, 0.918 0 g•L⁻¹; and the alcohol extract is the best method to extract antioxidative components, with the highest antioxidant activity and lowest IC₅₀. When the mass concentration of the herbs reached a certain degree, its free radical clearance rate was similar to that of vitamin C control group. The order of different chemical contributions of constituents to the antioxidant activity in the fingerprint was 4>3>33>53>9>10>11>34>15>59>8>61>52>20>42>18>29. The preliminary exploration for the spectrum efficiency relations provides reference for studying traditional Chinese medicine compound processing method and the pharmacodyamic material basis.
ABSTRACT
Objective: To investigate the factors significantly affecting the shoot buds regeneration of Carthamus tinctorius from Jimsar (CTJ). Methods: Through tissue culture experiments, the influences of culture temperature, illuminance, relative humidity, explants type, seedlings age, and plant growth regulators added in culture media on in vitro regeneration of CTJ were investigated. Results: The culture temperature was set at 24 °C in daytime and 16 °C at night, illuminance at 9000 lx, and 60% relative humidity were suitable for CTJ regeneration. Cotyledons excised from 6-8 d old seedlings were more responsive than even-aged euphyll, hypocotyl, and root explants because of inducing adventitious buds. The highest percentage of regenerated shoots (79.1%) with about five adventitious buds per responding explant was obtained from MS basal medium containing 12.0 mg/L TDZ, 2.5 mg/L IBA, and 1.5 mg/L 2-ip (No. 14 medium). Regenerated shoot buds (80%) could elongate successfully after 1-2 weeks transferred to shoot elongation medium. Conclusion: Suitable factors of shoot buds regeneration for CTJ were determined. It may also make a useful reference for regeneration researches of other C. tinctorius varieties in China. © 2014 Tianjin Press of Chinese Herbal Medicines.
ABSTRACT
High-performance liquid chromatographic coupled with variable wavelength detection (HPLC-VWD) has been developed for simultaneous determination of 5 analytes including ellagic acid, quercetin, kaempferol-3-O-beta-D-rutinoside, tiliroside and kaempferol, and high-performance liquid chromatographic with an evaporative light scattering detector (HPLC-ELSD) has been established to determine goshonoside-F5 in extract of Rubi Fructus. Chromatographic separations were carried out on an Agilent ZORBAX SB-C18 column (4.6 mm x 250 mm, 5.0 microm). All calibration curves of reference standards revealed good linearity (R2 > 0.999 5) within the concentration ranges tested. The method limits of detection ranged 0.297-90.144 ng and the method limits ofquantitation ranged 0.990-300.480 ng, respectively. Recoveries of 6 analytes were from 97.11% to 101.7%, with RSD less than 2.1%. The result shows that amounts of the 6 analytes in the samples from 16 localities were found to be different. The higher latitude of growing environment, the more ellagic acid in herb. The content of total flavonoids in sample from east localities were higher than that in middle and west localities, and the content of goshonoside-F5 in Bozhou, Anhui province was higher than others. This method was found to be simple, accurate, sensitive with good repeatability. Those results might serve as a sound foundation for further study, quality control and application of Rubi Fructus.